Workshops
All workshops will be held on Saturday, July 24, 2010. Pre-registration is mandatory and should be indicated on your registration form.
WK1: Global Phasing Software Suite for Macromolecular Crystallography
Saturday, July 24, 2010
Early Registration: $135 per person, Late Registration: $170 per person
Gerard Bricogne, Organizer
More information coming soon.
WK2: PLATON
Saturday, July 24, 2010
Early Registration: $140 per person, Late Registration: $175 per person
Lee Daniels, Organizer
More information coming soon.
WK3: Sulfur-SAD Data Collection and Phasing
Saturday, July 24, 2010, Univ. of Illinois at Chicago
Early Registration: $120 per person; Late Registration: $150 per person
This workshop (lectures in the morning and hands-on exercises in the afternoon) is intended for both students and professionals interested in SAD data collection and phasing. The focus of the workshop will be on sulfur-SAD phasing mainly because it is the most demanding SAD phasing technique in terms of sample preparation, crystal mounting, data collection and data processing.
Morning session lecturers will cover such topics as (1) SAD theory, (2) Sample preparation, (3) Source/beamline considerations, (4) Data collection strategies, (5) Data processing theory, (6) Anomalous substructure determination and (7) Generating initial protein phases and electron density maps.
The afternoon session will focus on hands-on exercises in data reduction and sulfur-SAD phasing. Here attendees will process a set of zinc-free insulin data (51 residues, 3 disulfides, space group I213, a = 78Å) using common data reduction packages. Then, using the scaled structure factors, attendees will attempt to (1) determine the anomalous scattering substructure (insulin disulfide centroids), (2) determine the correct hand of the disulfide centroids, (3) calculate a set of initial protein phases and (4) interpret the quality of the electron density map produced.
Attendees will also work on the more demanding sulfur-SAD structure determination of ORF 1382 from Archaeoglobus fulgidus (95 amino acids, 5 sulfurs, space group P42) whose crystals only diffract to 2.65Å resolution.
All workshop attendees will receive a DVD containing lecture materials and a how-to guide for the crystallization of zinc free insulin and crystal cryoprotection protocol together with instructions for crystal mounting and data collection so that they can practice sulfur-SAD data collection and phasing at their home institution, after the workshop.
Workshop instructors
Bi-Cheng Wang, University of Georgia
John P. Rose, University of Georgia
John Chrzas, SER-CAT Advanced Photon Source, Argonne National Laboratory
Zeng-Qing Fu, SER-CAT Advanced Photon Source, Argonne National Laboratory
Bernard Santarsiero, University of Illinois at Chicago
Manfred Weiss, European Molecular Biology Laboratory, Hamburg, Germany
James Liu, Institute of Biophysics, Academia Sinica, Beijing, China
WK4: Getting the Most out of the CCP4 Suite
Saturday, July 24, 2010
Early Registration: $120 per person; Late Registration: $150 per person
Charles Ballard, Organizer
More information coming soon.
WK5: Crystallography: World of Wonders
Saturday, July 24, 2010
Please contact aca@hwi.buffalo.edu if you are interested in attending this workshop.
Cora Lind, Organizer, with Christine Zardecki, David Goodsell, Claudia Rawn, and Cora Lind for Chicago Public School teachers
More information coming soon.
